C-flat holey carbon coated grids for cryo-TEM

C-flat is an ultra-flat, holey carbon-coated TEM support grid for transmission electron microscopy (TEM). Unlike competing holey carbon films, C-flat is manufactured without plastics, so it is clean upon arrival and the user has no residue to contend with.

Made with patent pending technology, C-flat provides an ultra-flat surface that results in better particle dispersion and more uniform ice thickness. Patterning is done using deep-UV projection lithography, ensuring the most accurate and consistent hole shapes and sizes down to submicron features. The precise methods by which C-flat is manufactured eliminate artefacts such as excess carbon and edges around holes.

 C-flat holey carbon film has carbon 20nm.  

C-flat holey carbon grids provide the ideal specimen support to achieve high resolution data in cryo-TEM making them an ideal choice for single particle analysis, cryo electron tomography and automated TEM analysis.

Cryo-Electron Tomography (cryoET) and Single Particle Analysis ( SPA ):

Numerous researchers have reported that the ultra-flat surface of C-flat? leads to even ice thickness and uniform particle distribution within the hole areas. This optimal particle distribution results in superior data being collected as compared with other holey support films. 2um hole sizes are standard.

Automated TEM

C-flat? provides a regular array of analysis sites compatible with automated data collection software such as Leginon. This compatibility, in combination with the more uniform ice thickness and particle distribution reported by numerous researchers, results in more high-quality target sites per grid.

Product Line

C-flat is a holey carbon film supported by a standard TEM grid. C-flat products are fully specified by 4 parameters: the hole diameter and pitch of the holey carbon film and the material type and mesh size of the TEM grid. The following image illustrates these parameters

Standard Products

The breadth of applications in cryoTEM necessitate a wide range of holey carbon film patterns. And now, with the recent expansion of the product line, a C-flat holey carbon film is available for almost any application. Whether 600nm holes are needed for very high magnifications with ultra-high resolution cameras or large open areas are needed for larger specimens, C-flat? is the perfect holey carbon grid.

C-flat is immediately available in several standard array patterns

First number = Hole diameter (1.2um)

Second number = Hole spacing (1.3um)

Including hole diameters/hole spacings of 0.6/2, 1/1, 1/2, 1/4, 1.2/1.3, 2/1, 2/2, 2/4, 4/2, and a multihole pattern. C-flat is supported by your choice of a 200 mesh or 400 mesh copper TEM grid and sold in quantities of 25, 50, or 100.

Thick Products

C-Flat is available in a thick option that doubles the carbon thickness from approximately 20nm to 40nm. Thick C-flat product numbers end in -T, catalog numbers contain "CFT". Available in quantities of 50 and 100 per pack.