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Citrate buffer
This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZEÂ
Intended Use:Â 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 6 antigen retriever solution.
Preparation of Working Solution: Dilute this 10x buffer as needed (e.g. 90 ml of deionised or distilled water + 10ml of this buffer), mix well, 1x buffer can be stored at 2-8°C. 2
Code | Title | pH | Pack Size | Availability | Price | Updated: 28-04-2024 |
---|---|---|---|---|---|---|
EMS64142-07 | Citrate buffer | 6 | 100ml | See above | $169.00 AUD | |
EMS64142-08 | Citrate buffer | 6 | 1L | See above | $281.00 AUD | |