Basic blocking conjugates solutions

AURION Blocking Solutions are prepared using specially selected compounds. All ruminant proteins are obtained from healthy livestock.

AURION Blocking solutions contain Bovine Serum Albumin and Cold Water Fish Skin Gelatine in phosphate buffered saline with sodium azide as preservative. Normal serum may have been added as indicated on the label. The blocking capacity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods 74, (1984), 353.

Specificity

Blocking Solutions are available in the following specificities:
serum-free: for use with Protein A and Protein G Gold conjugates.
with Normal Goat serum: for use with reagents based on secondary antibodies raised in Goat.
with Normal Rabbit serum: for use with reagents based on secondary antibodies raised in Rabbit.
with Normal Sheep serum: for use with reagents based on secondary antibodies raised in Sheep.
with Normal Donkey serum: for use with reagents based on secondary antibodies raised in Donkey.

Technical Tips

(Electron Microscopy)
 On-Grid Labeling :Placing grids on top of drops of Blocking Solution arrayed on a sheet of Parafilm? after they have been treated for aldehyde inactivation.
Pre-embedding Labeling: Floating specimens in Blocking Solution, preferably on a rocking table.
(Light Microscopy) 
On-slide or -coverslip Labeling Applying a few drops of Blocking Solution to cover the specimen.
 - Bio Assays Incubating specimens (e.g. immunoblot strips) in Blocking Solution on a rocking table in screw-cap sealed disposable tubes.

Storage

AURION Blocking Solutions have a guaranteed shelf life of 18 months from the date of quality control analysis. The products should be stored at 4-8°C. Freezing is not recommended.