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Prices are current as of Tuesday, 12/11/2019.
Prices are GST exclusive. Tax is added at checkout.

$1,970.00

NOTICE: This price is only an estimate and will be changed when a quote is requested.

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Product Code: OSFA-CY

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Adapt your existing lab stereo microscopes for fluorescence

The NIGHTSEA stereo microscope fluorescence adapter adapts just about any stereo microscope (dissecting microscope) for fluorescence with no modification to the microscope itself. The modular design lets you easily switch between several different excitation/emission combinations to work with a variety of fluorescent proteins and other fluorophores. There are now six different excitation/emission combinations available, plus white light.

Applications

This simple system is excellent for:

  • Quick screening of your fluorescent genotypes -Drosophila, zebrafish, C. elegans,...
  • Genotype sorting
  • Fluorescence-aided dissection, injection, or micromanipulation
  • Freeing up your research-grade fluorescence microscopes for more demanding work
  • New faculty start-up budgets
  • Bringing fluorescence into the teaching laboratory

Mouse with actin labeled with GFP. Photo © NIGHTSEA/Charles Mazel
Mouse with actin labeled with DsRed. Photo © NIGHTSEA/Charles Mazel
GFP-tagged Drosophila larva. Photo © NIGHTSEA/Charles Mazel

Fluorescence isn't just for research microscopes anymore

  • Now sort on your laboratory-level stereos
  • Use to facilitate micromanipulation and dissection
  • Expand from your research lab to your classroom

Modular

  • Installs in seconds - just clicks into place
  • Interchangeable excitation/emission combinations
  • Move from microscope to microscope
  • No modification to your micoscope needed

Economical - more glow for the dough

  • Stretch your lab budget
  • Inexpensive enough for classroom use

Grows as your lab grows

  • Buy just what you need now (up to 5 different wavelength sets)
  • Add more as your needs expand

The stereo microscope fluorescence adapter system consists of

  • Flexible gooseneck lamp base with power supply
  • Light head
  • Ring adapter for microscope
  • Barrier filter
  • Filter shield

The light head, barrier filter, and filter shield are interchangeable so that you can easily switch between excitation/emission light filter combinations.

The microscope mounting adapter fits up to 67mm to work with the majority of stereo microscopes.

Once you are set up for one excitation/ emission wavelength combination, additional combinations can be added by purchasing a kit that consists of a light head, barrier filter, and viewing shield. These three elements can be removed and replaced in seconds, and color coding ensures that you are using the right combination. The barrier filter clicks on to the ring adapter magnetically, so it is easy to remove it to switch back to white light viewing.

Specifications

Filter Set Excitation Emission Fluorophores
RB - Royal Blue 440-460nm 500nm LP GFP, eGFP, fluorescein...
RB-GO 440-460nm 500-560nm BP GFP, eGFP, fluorescein...
CY - Cyan 490-515nm 550nm LP YFP, Venus, Lucifer Yellow...
GR - Green 510-540nm 600nm LP DsRed, dTomato...
VI - Violet 400-415nm 460nm LP CFP, ...
UV - Ultra Violet 360-380nm 415nm LP DAPI, ...

Microscope Mounting Adapter - fits up to 67mm standard.

Green-only barrier filter

The green-only barrier filter isolates the green part of the spectrum and is for use with the Royal Blue excitation source. While our other barrier filters are long-pass filters this new filter is a bandpass, transmitting from approximately 500 to 560nm. The long-pass filter has served well for most users who need to visualize green-fluorescent protein (GFP), and if you are exploring fluorescence in nature it is preferable. The primary motivation for adding the green-only filter to the line-up was for the benefit of researchers using GFP in plants such as Arabidopsis thaliana, a common research model. Plants contain chlorophyll, which has a distinctive red fluorescence that can sometimes mask the GFP emission, making it harder to see and photograph.

GFP-tagged Drosophila larva. Photo © NIGHTSEA™/Charles Mazel
GFP-tagged Drosophila larva. Photo © NIGHTSEA™/Charles Mazel

NIGHTSEA adapter
NIGHTSEA adapter

NIGHTSEA microscope adapter
NIGHTSEA microscope adapter

Tech Tip: Will the Stereo Microscope Fluorescence Adapter Fit my Microscope?

Lamp Base Light Control Options

The NIGHTSEA lamp base is available in three versions: Standard, DIM, and PULSE. Only one control option is available per base. The DIM and PULSE options can be purchased as part of a new system, as a retrofit to an existing base, or in a stand-alone lamp base purchased a la carte.

NIGHTSEA Standard Option

Standard lamp bases provide a steady light when turned on. DIM lamp bases feature an OFF/ON/DIM switch to change the intensity of the light. PULSE lamp bases incorporate a BNC connector that accepts a voltage signal to control the excitation source ON/OFF.

Dimmer switch option on SFA

Target at max and min excitation 

NIGHTSEA DIM Option

The DIM option adds a combined switch and dimmer control. When you first turn the unit on it is at full power. As you rotate the switch the intensity decreases, reaching about 30% power at the minimum setting. You have finer control in the brighter portion of the adjustment range. An intensity indicator (1-10) makes it easy to record and repeat preferred settings.

BNC input connector for external controlBNC input connector for external control 

NIGHTSEA PULSE Option

The PULSE option adds a BNC connector on the rear of the base that accepts a user-supplied voltage input to turn the light on and off. Some possible applications are

  • External control (computer, function generator, etc.) for precise illumination timing for photoactivation, behavioral experiments
  • PWM input for light dimming
  • Footswitch for hands-free operation - contact EMS for details

Specifications

  • Voltage input - 2.8 - 6.0 VDC for ON, <0.6VDC OFF
  • Maximum operation frequency - 10kHz
  • BNC cable not included

Shipping

Orders with items in stock are packed within 24 hours. We ship with our best choice of courier, depending on goods ordered and shipping address provided.

Location

Standard delivery

Express delivery

Townsville/Local

Next day

N/A

Queensland

2-3 business days

N/A

NSW

4-10 business days

2-3 business days

SA/Tas/Vic/WA

6-14 business days

2-3 business days

Overseas

N/A

5-14 business days

*Note: Delivery times are as provided by our carriers and are estimates only. Delays may occur during peak periods, and due to factors outside the carriers' control.

Standard shipping is $15 in Australia (free on orders over $200). Add $5 to ship Express (under 2kg only)

Pickup

Order online and collect from our warehouse in Townsville.

Location

Freight Charge

Ready for collection

11 Carlton Street, Kirwan

$0

Once invoice has been emailed
(Less than 1 business day)



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