JB-4 embedding kit (DG)
A water soluble embedding media which is based on Glycol Methacrylate (GMA) plastic embedding. It is intended for use in preparing samples for high resolution microscopes (HRLM). The catalysed monomer acts as a dehydration and infiltration agent; therefore, complete dehydration through 100% ethanol is not necessary (although recommended for large or dense tissues). Conventional paraffin sections have a greater degree of shrinkage and produce inadequate morphology when compared to JB-4. Features:
Thin sections (.5-2.0 micron) with excellent morphological structure preservation. Water-clear blocks; casts in 90 minutes max. at room temperature. Good lipid and enzyme retention when processing at low temperatures (4°C). Removal of JB-4 resin prior to staining is not necessary. Clearing agents such as xylene and chloroform are not needed. Higher clarity and contrast than with paraffin sections. Easy processing of difficult specimens such as calcified bone and delicate embryonic tissue. All components may be stored at room temperature.
References: 1.Hofman E.O. & Flores, T.R., High Resolution LM in Renal Path., Amer. J. of Clin. Path., 76:5 (1981) 2.Beckstead, J.H., Blood, 57(6):1008 (1981) 3.Brinn, N & Pickett, P., J. Histotech., 2(5):125-130 (1979) 4.Block, Matthew H., et al., Lab. Med., 13(5)(1982) 5.Helander, K.G., J. Microscopy, 132:223 (1983) 6.Cole, M.B., J. Microscopy, 127:139 (1982) 7.Higuchi, S., et al., Stain Tech., 54(1):5-12 (1979) 8.Van DeVeldt, S., Am. J. Clin. Path.,73:121 (1980) 9.Horton, W.A., J. Histochem. Cytochem., 31:417 (1983) 10.Tacha, D.E. & Richard, T.C.., J. Histotech., 4(2):59 (1981)
C611 JB-4 solution A only, 750ml MSDSC612 JB-4 solution B only 50ml MSDSC613 JB-4 catalyst 12g MSDS
JB-4 kit components: