Coverglass bottom culture dishes, sterile

A new series of sterile, optical quality, glass bottom dishes which provide exceptional imaging quality for many applications requiring the use of inverted microscopes. 

High quality thin glass bottom sterile culture dishes.

Ideal For

• Fluorescence microscopy
• High resolution image analysis
• Electrophysiology
• Confocal (laser) microscopy
• Cell culture

Conventional plastic or glass Petri dishes and chambers limit the use of the inverted microscope for many applications because the thick plastic or glass bottom requires a long working distance objective available only in lower magnifications. Moreover, plastic dishes cannot be used for DIC or any polarisation microscopes due to their inherent birefriengence.
Thin glass bottom dishes eliminate these problems, making them ideal for applications such as: patch clamp recordings where fluorescent-tagged (GFP) receptors can be identified for selective study; and many other fluorescent microscopy applications, which require low background scattering of light and reduce intrinsic "auto" fluorescence that occurs when using standard plastic dishes.
Furthermore, the glass bottom is designed flush with the whole base of the dish, therefore allowing direct contact between the entire glass surface and the warming stage. This eliminates any air gaps that may otherwise exist between the glass dish and the warming stage and hence optimise heat transfer, allowing homogenous heating and cooling of the glass bottom.

Features:

• Non-cytotoxic, non-pyrogenic and sterile until opened.
• Dish diameters: 35 and 50mm, with internal glass well diameter of 12, 22, 30, and 40mm.
• Each dish has a flat (0.17mm thick) optical quality glass bottom.
• Production is carried out in a Class 10,000 clean room, certified under EN46001, EN46002, ISO9001 and ISO9002.
• All dishes are gamma irradiated (EN552) and sterile.

Optical Properties:

• Wide optical range of transmission- UV, IV, and IR (300nm to 2500nm).
• Flat (0.17mm thick) optical quality glass bottom provides the following advantages:
• Use of a much shorter working distance immersion objective.
• Larger numerical aperture (NA) objectives.
• Higher magnification (up to 100x)

Microscopy, Imaging, and Spectroscopy:

• Confocal (laser) microscopy.
• Two-/multi photon confocal microscopy.
• DIC and polarisation microscopy.
• High-resolution imaging system.
• Infrared imaging (e.g. brain slide recordings).

Some Applications:

• Cell Biology: stem cell research, cell growth cycle monitoring, cell culture.
• Protein chemistry: green fluorescent protein identification of histotags, etc.
• Molecular biology: phenotyping and other complex genetic research.

The catalogue number indicates the size of the dish and well:
H351012 = dish diam. 35mm, height 10mm, well diam. 12mm.
The dishes are bulk packed in plastic sleeves of 20. Also available individually blister-packed at a higher price - ask